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2011•Journal of Instrumental AnalysisRequires access

Investigation on the Isolation and Purification of EGCG3″Me by High Speed Countercurrent Chromatography

Jiwei Chen

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Abstract

A method for the isolation and purification of(-)-epigallocatechin-3-(3″-O-methyl)gallate(EGCG3″Me) from crude extract sample was firstly developed by high speed countercurrent chromatography(HSCCC).The results showed that HSCCC had a two-phase solvent system composing of water-methanol-ethyl acetate-hexane(5 ∶2 ∶9 ∶1,by volume) on a preparative scale.The upper phase was used as the stationary phase,and the lower phase was used as the mobile phase.The revolution speed was 700 r/min,the flow rate was 2.0 mL/min and the detection wavelength was set at 254 nm.Under the optimized conditions,EGCG3″Me can be well isolated.The purity of EGCG3″Me detected by high performance liquid chromatography was more than 90%,and the yield of EGCG3″Me by HSCCC was 76.53%.

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What this paper is about

A method for the isolation and purification of(-)-epigallocatechin-3-(3″-O-methyl)gallate(EGCG3″Me) from crude extract sample was firstly developed by high speed countercurrent chromatography(HSCCC).The results showed that HSCCC had a two-phase solvent system composing of water-methanol-ethyl acetate-hexane(5 ∶2 ∶9 ∶1,by volume) on a preparative scale.The upper phase was used as the stationary phase,and the lower phase was used as the mobile phase.The revolution speed was 700 r/min,the flow rate was 2.0 mL/min and the detection wavelength was set at 254 nm.Under the optimized conditions,EGCG3″Me can be well isolated.The purity of EGCG3″Me detected by high performance liquid chromatography was more than 90%,and the yield of EGCG3″Me by HSCCC was 76.53%.

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Available abstract

A method for the isolation and purification of(-)-epigallocatechin-3-(3″-O-methyl)gallate(EGCG3″Me) from crude extract sample was firstly developed by high speed countercurrent chromatography(HSCCC).The results showed that HSCCC had a two-phase solvent system composing of water-methanol-ethyl acetate-hexane(5 ∶2 ∶9 ∶1,by volume) on a preparative scale.The upper phase was used as the stationary phase,and the lower phase was used as the mobile phase.The revolution speed was 700 r/min,the flow rate was 2.0 mL/min and the detection wavelength was set at 254 nm.Under the optimized conditions,EGCG3″Me can be well isolated.The purity of EGCG3″Me detected by high performance liquid chromatography was more than 90%,and the yield of EGCG3″Me by HSCCC was 76.53%.

Key concepts: Countercurrent chromatography, Chemistry, Chromatography, Stationary phase, Yield (engineering), Countercurrent exchange, Phase (matter), Solvent

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