Enzymatic Extraction of Collagen from Poultry Bone by Pepsin

Enzymatic treatment to improve the extraction of collagen from chicken bone powder was explored in this paper.The raw materials was decalcified by 1.0mol/L HCl for 2 days,defatted by hexane and then hydrolyzed by pepsin under the following optimal conditions:enzyme dosage of 150U/g,reaction time of 120min,pH value of 1.7,and reaction temperature of 37℃.For the extraction of collagen from the enzymatic hydrolyzate,the best liquid/solid ratio and extraction time were 6:1 and 5h respectively.Under these conditions,the extraction rate and protein content of the collagen from poultry bone were of 18.4% and 54%,respectively.