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Optimization of Extraction Process of Collagen from Pigskin by Enzyme Hydrolysis

Hui Wang

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Abstract

Objective:Screen the optimum preparation for collagen by enzyme hydrolysis.Method: Enzy-matic condition was optimized by single-factor experiments and the orthogonal design with collagen yield as the in-dex.Result: The optimum extraction condition was obtained as follows:the ratio of enzyme /substrate was 3%,pH 2.0,material-liquid ratio 1∶ 28,enzymatic hydrolyzed for 48 h at 0 ℃.Conclusion:Preparation for pigskin collagen by pepsin is better.

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Objective:Screen the optimum preparation for collagen by enzyme hydrolysis.Method: Enzy-matic condition was optimized by single-factor experiments and the orthogonal design with collagen yield as the in-dex.Result: The optimum extraction condition was obtained as follows:the ratio of enzyme /substrate was 3%,pH 2.0,material-liquid ratio 1∶ 28,enzymatic hydrolyzed for 48 h at 0 ℃.Conclusion:Preparation for pigskin collagen by pepsin is better.

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Available abstract

Objective:Screen the optimum preparation for collagen by enzyme hydrolysis.Method: Enzy-matic condition was optimized by single-factor experiments and the orthogonal design with collagen yield as the in-dex.Result: The optimum extraction condition was obtained as follows:the ratio of enzyme /substrate was 3%,pH 2.0,material-liquid ratio 1∶ 28,enzymatic hydrolyzed for 48 h at 0 ℃.Conclusion:Preparation for pigskin collagen by pepsin is better.

Key concepts: Hydrolysis, Extraction (chemistry), Yield (engineering), Chromatography, Chemistry, Enzyme, Enzymatic hydrolysis, Substrate (aquarium)

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