Extraction of Genomic DNA in Pistacia Chinesis Bunge

It is difficult to extract genomic DNA due to the existence of phenolic compounds,pigments,polysaccharides,and other secondary metabolites in the leaves of Pistacia chinesis Bunge.In order to obtain its high-quality genomic DNA,CTAB method and improved CTAB method were used to extract its genomic DAN,and then the quality of extracted DNA were detected by qualitative analysis,quantitative analysis and PCR analysis.The tesults showed that the extracted DNA by improved CTAB method were clear and had no RNA contamination by the electrophoresis.The ratio of OD260 to OD280 was around 1.8.RAPD bands amplified were clear and had non-diffusin phenomena.The DNA isolated by improved CTAB method is suitable for PCR amplification.