2006•Forest Research Open AccessRequires access

Extract Genomic DNA from Phyllostachys edulis by CTAB-Based Method

Gao Zhimin, Xueping Li, Chunju Cai, Peng Zhen-hua, Sfa Key

Abstract

CTAB method,improved CTAB method,improved CTAB-high salt precipitation method were used to extract genomic DNA from the leaves of Ph.edulis.The DNA samples obtained were tested by UV spectrophotometer(3 300),agarose gel electrophoresis and PCR method.The differences in yield,quality and PCR result of the obtained DNA were compared.The results revealed that improved CTAB-high salt precipitation method was an inexpensive and reliable method for the genomic DNA extraction of Ph.edulis.

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Key concepts: Phyllostachys edulis, genomic DNA, Chromatography, Agarose gel electrophoresis, DNA, DNA extraction, Chemistry, Agarose