Study on Genomic DNA Extraction Method for Bletilla striata

Objective: By comparing different methods for genomic DNA extraction,the best genomic DNA extraction method of Bletilla striata,suitable for DNA barcoding,was selected. Method: CTAB method,SDS method,alkaline lysis method and improved CTAB method were used to extract genomic DNA from B. striata.The yield and quality of genomic DNA were evaluated by Agarose gel electrophoresis,UV-spectrophotometry and PCR. Result: CTAB method,SDS method and improved CTAB method could extract genomic DNA from B.striata,while alkaline lysis method yields little. The quality of genomic DNA yielded by improved CTAB method were higher than those yielded by other three methods,and its amplification success rate was 100 % with clear electrophoretic bands of amplified products. Conclusion: The improved CTAB method was the best method for genomic DNA extraction from B. striata and could satisfy the need for DNA barcoding study.