Expression and Significance of Survivin and PCNA in Human Colorectal Carcinoma

Objective To evaluate the expression and significance of survivin and PCNA in human colorectal carcinoma. Methods Using in situ hybridization and immunohistochemistry(S-P method) respectively to detect survivin mRNA, and survivin and PCNA proteins in 64 cases of colorectal carcinoma. Results The survivin expression wasn't detected in the tumor adjancent tissues. The positive rate of survivin mRNA and protein expressions was 53% and 53% respectively, which was significantly associated with the histological type and lymph node metastasis of colorectal carcinoma (P0.05). PCNA labelling index in the colorectal carcinoma was 33.65±18.7%. There was a positive correlation between survivin and PCNA protein expressions in the colorectal carcinomas. Conclusion Survivin expression increased in colorectal carcinoma,which was related to histological type,lymph node metastasis and proliferation potential of colorectal carcinomas. Survivin may be a new diagnostic/therapeutic target of human colorectal carcinoma.