Cloning and Sequencing of gE Gene Encoding Area of Pseudorabies Virus Fa Strain Excluding Signal Peptide

The gE gene encoding area of pseudorabies virus Fa strain excluding signal peptide was amplified by PCR from virus genome DNA.This PCR product was cloned into pMD18-T vector and then sequenced.The sequencing result showed that the recombinant plasmid contained the gE gene encoding area of PRV Fa strain excluding signal peptide.The identity of nucleotide sequence and amino acid sequence of this region between PRV Fa strain and Rice strain was 97.5% and 94.8%,respectively.