2001•Zhongguo yufang shouyi xuebaoRequires access
The Construction of Recombinant Plasmid Containing gE Gene Epitope Encoding Area of Pseudorabies Virus Fa Strain
AO Jingqun
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Abstract
The gE gene epitope encoding area of pseudorabies virus Fa strain was amplified by PCR from template plasmid pMD18T_FS.Then the PCR products were cloned into pPICZaA and pAcGP67A vector respectively.The recombinant plasmids pPICZaA_FS and pAcGP67A_FS were identified by double restriction endonuclease digestion and DNA sequence analysis.The sequencing results showed that the recombinant plasmid contained the gE gene epitope encoding area of PRV Fa strain.