Cloning and sequencing of gE gene fragment exclude signal peptide of pseudorabies virus Guangdong strain

A fragment about 1.6 kb was amplified by PCR technique from the genome DNA of PRV Guangdong strain,using primers designed and synthesized according to the gE gene fragment exclude signal peptide of PRV Rice strain. This PCR product was then cloned into pMD18-T vector and sequenced. The sequencing result showed that the PCR product spanned 1 665 bp,encoding 555 amino acids. The homology of DNA sequence and amino acid sequence between it and gE gene of Rice strain was 97.7% and 95.9% respectively.