Cloning and Sequencing of gE Gene Fragment Encoding Epitopes of Pseudorabies Virus Fa Strain

The gE gene fragment encoding epitopes of pseudorabies virus Fa strain was amplified by PCR.This PCR product was cloned into pMD18-T vector and then sequenced.The sequencing result showed that the recombinant plasmid contained the epitopes encoding area of PRV Fa strain.The identity of DNA sequence and amino acid sequence of this region between PRV Fa strain and Rice strain was 97.3% and 94.7% respectively.