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2008•Anhui Medical and Pharmaceutical JournalRequires access

Determination of puerarin in xiaoke pill by HPLC

Chuanshan Jin

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Abstract

Aim To determine puerarin in xiaoke pill by HPLC.Methods HPLC was used in the quantitative analysis of Puerarin in the sample.The separation was performed on Hanbon C18(4.6 mm×250 mm,5 μm)column with a mixture of acetonitrile-0.5% phosphonic acid(15∶85) as mobile phase.The detection wavelength was set at 250 nm and the flow rate was 1.0 ml·min-1.Results The standard curve was linear in the range befween 23.0 and 138.0 mg·L-1 for puerarin(r=0.999 6,n=5).The average recovery was 97.19%.Conclusion The method is simple,reliable,and is a good method to determine puerarin.

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What this paper is about

Aim To determine puerarin in xiaoke pill by HPLC.Methods HPLC was used in the quantitative analysis of Puerarin in the sample.The separation was performed on Hanbon C18(4.6 mm×250 mm,5 μm)column with a mixture of acetonitrile-0.5% phosphonic acid(15∶85) as mobile phase.The detection wavelength was set at 250 nm and the flow rate was 1.0 ml·min-1.Results The standard curve was linear in the range befween 23.0 and 138.0 mg·L-1 for puerarin(r=0.999 6,n=5).The average recovery was 97.19%.Conclusion The method is simple,reliable,and is a good method to determine puerarin.

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Available abstract

Aim To determine puerarin in xiaoke pill by HPLC.Methods HPLC was used in the quantitative analysis of Puerarin in the sample.The separation was performed on Hanbon C18(4.6 mm×250 mm,5 μm)column with a mixture of acetonitrile-0.5% phosphonic acid(15∶85) as mobile phase.The detection wavelength was set at 250 nm and the flow rate was 1.0 ml·min-1.Results The standard curve was linear in the range befween 23.0 and 138.0 mg·L-1 for puerarin(r=0.999 6,n=5).The average recovery was 97.19%.Conclusion The method is simple,reliable,and is a good method to determine puerarin.

Key concepts: Puerarin, Chromatography, High-performance liquid chromatography, Chemistry, Linear range, Pill, Calibration curve, Acetonitrile

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