HPLC Fingerprints of Radix Codonopsis Processed Products

Objective To study the HPLC fingerprints of 3 kinds of Radix Codonopsis processed products and to provide a responsible processing approach for this herb.Methods By using HPLC-DAD,this study compared and analyzed Radix Codonopsis from 10 different localities and its intermediate material and end product.The separation was performed on a Phenomenex C18 column(250mm×4.6mm,5 μm) with linear gradient elution.The mobile phase consisted of 0.05% phosphoric(5) acid solution and acetonitrile at the flow rate of 0.8 ml·min-1.The column temperature was at 30 ℃,UV detection wavelength was at 268 nm.Results By a special system named Extendable Data base for Quality Assessment of Traditional Chinese Herbal Medicine,samples were examined to generate a mean global chromatogram and the similarity among Radix Codonopsis slice,intermediate material and end product was also calculated.Conclusion The similarity was 0.9814±0.0130(mean±standard),and the similarities and differences of the 3 kinds of samples were analyzed.This method shows the stability of Radix Codonopsis from 10 different places and can provide scientific basis for Radix Codonopsis purchasing and processing.