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2012•Journal of Jiangxi University of Traditional Chinese MedicineRequires access

HPLC Fingerprint Investigation on Dried Rehmanniae Radix

Qionglin Liang

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Abstract

Objective: To establish HPLC fingerprint of dried Rehmanniae Radix and provide scientific reference for the quality con trol.Methods: A Grace Allitma C18 column(250mm×4.6mm,5μm) was employed and the acetonitrile-water was used as mobile phase.The detective wavelength was 203nm and analytic time 45min.The data analysis of retention time and peak area of the HPLC fin gerprints was performed with a similarity evaluation system developed by China Pharmacopoeia Committee.Result: The standard HPLC fingerprint spectrum of dried Rehmanniae Radix was successfully established based on 13 batches of products.Eleven common peaks were pointed out and the similarity of 13 batches of dried Rehmanniea Radix was between 0.849 and 0.987.Conclusion:The es tablished method is stable and reliable and can provide much information for the quality control of dried Rehmannia Radix.

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What this paper is about

Objective: To establish HPLC fingerprint of dried Rehmanniae Radix and provide scientific reference for the quality con trol.Methods: A Grace Allitma C18 column(250mm×4.6mm,5μm) was employed and the acetonitrile-water was used as mobile phase.The detective wavelength was 203nm and analytic time 45min.The data analysis of retention time and peak area of the HPLC fin gerprints was performed with a similarity evaluation system developed by China Pharmacopoeia Committee.Result: The standard HPLC fingerprint spectrum of dried Rehmanniae Radix was successfully established based on 13 batches of products.Eleven common peaks were pointed out and the similarity of 13 batches of dried Rehmanniea Radix was between 0.849 and 0.987.Conclusion:The es tablished method is stable and reliable and can provide much information for the quality control of dried Rehmannia Radix.

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Available abstract

Objective: To establish HPLC fingerprint of dried Rehmanniae Radix and provide scientific reference for the quality con trol.Methods: A Grace Allitma C18 column(250mm×4.6mm,5μm) was employed and the acetonitrile-water was used as mobile phase.The detective wavelength was 203nm and analytic time 45min.The data analysis of retention time and peak area of the HPLC fin gerprints was performed with a similarity evaluation system developed by China Pharmacopoeia Committee.Result: The standard HPLC fingerprint spectrum of dried Rehmanniae Radix was successfully established based on 13 batches of products.Eleven common peaks were pointed out and the similarity of 13 batches of dried Rehmanniea Radix was between 0.849 and 0.987.Conclusion:The es tablished method is stable and reliable and can provide much information for the quality control of dried Rehmannia Radix.

Key concepts: Radix (gastropod), High-performance liquid chromatography, Pharmacopoeia, Chromatography, Fingerprint (computing), Traditional medicine, Chemistry, Medicine

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