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2009•The Chinese Journal of Modern Applied PharmacyRequires access

Study on the HPLC Fingerprint of Radix Scutellariae and Its Processed Pieces

Runtao Tian

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Abstract

OBJECTIVE To study the HPLC fingerprints of Radix Scutellariae and its processed pieces.METHODS Selecting HPLC-UV fingerprints.Using a Hypersil C18 column(200 mm×5.0 mm,5 μm).The mobile phase was linear gradient elute with methanol-0.4% phosphoric acid-acetonitrile.Flow rate was 1.0 mL·min-1.Column temperature was 28 ℃.Detection wavelength was set at 277 nm.RESULTS There was no evident difference among the fingerprints of 10 batches of Radix Scutellariae that were normatively manufactured and processed,and the similarity of 14 batches of Radix Scutellariae was very well.CONCLUSION This method recurs well,and can be used to provide scientific basis for Radix Scutellariae and its processed pieces quanlity control mode.

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What this paper is about

OBJECTIVE To study the HPLC fingerprints of Radix Scutellariae and its processed pieces.METHODS Selecting HPLC-UV fingerprints.Using a Hypersil C18 column(200 mm×5.0 mm,5 μm).The mobile phase was linear gradient elute with methanol-0.4% phosphoric acid-acetonitrile.Flow rate was 1.0 mL·min-1.Column temperature was 28 ℃.Detection wavelength was set at 277 nm.RESULTS There was no evident difference among the fingerprints of 10 batches of Radix Scutellariae that were normatively manufactured and processed,and the similarity of 14 batches of Radix Scutellariae was very well.CONCLUSION This method recurs well,and can be used to provide scientific basis for Radix Scutellariae and its processed pieces quanlity control mode.

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Available abstract

OBJECTIVE To study the HPLC fingerprints of Radix Scutellariae and its processed pieces.METHODS Selecting HPLC-UV fingerprints.Using a Hypersil C18 column(200 mm×5.0 mm,5 μm).The mobile phase was linear gradient elute with methanol-0.4% phosphoric acid-acetonitrile.Flow rate was 1.0 mL·min-1.Column temperature was 28 ℃.Detection wavelength was set at 277 nm.RESULTS There was no evident difference among the fingerprints of 10 batches of Radix Scutellariae that were normatively manufactured and processed,and the similarity of 14 batches of Radix Scutellariae was very well.CONCLUSION This method recurs well,and can be used to provide scientific basis for Radix Scutellariae and its processed pieces quanlity control mode.

Key concepts: Chromatography, Radix (gastropod), High-performance liquid chromatography, Gradient elution, Chemistry, Phosphoric acid, Fingerprint (computing), Computer science

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Study on the HPLC Fingerprint of Radix Scutellariae and Its Processed Pieces — Research Paper | ScholarLens