Determination of paeoniflorin and baicalin in Qizhixiangfu Pills by HPLC

Objective To establish an HPLC method for the determination of paeoniflorin and baicalin in Qizhixiangfu pills. Methods The analysis was carried out on a Capcell PAK C18column( 4. 6 mm × 250 mm,5μm). The mobile phase was acetonitrile( A)- 0. 2% phosphoric acid( B) with gradient elution and the detection wavelength was 230 nm( 0 ~ 5 min,80% B;5 ~ 15 min,80% B→50%);280 nm(15 ~22 min,50% B→20%;22 ~26 min,20%B) and the flow rate was 1 mL·min- 1;The column temperature was 30 ℃. Results The linear ranges of paeoniflorin and baicalin were 0. 40 ~ 4. 76 μg and 0. 66~ 7. 97 μg,respectively. The average recoveries were 98. 42%( RSD = 0. 83%) for paeoniflorin and 99. 10%( RSD =1. 16%) for baicalin. Conclusion The method was simple,stable accurate and repeatability,and can be used for determining paeoniflorin and baicalin in Qizhixiangfu pills.