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Simultaneous HPLC determination of paeoniflorin and baicalin in Kuijiekang pepllets

Yajun Zhang, Zheng Hang-shenga

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Abstract

Objective:To establish a method for the determination of paeoniflorin and baicalin in Kujiekang peplle- ts.Methods:The HPLC method was conducted on a Kromasil C_(18)column(250 mm×4.6 mm,5.0 μm).The mobile phase consisted of 0.2% phosphoric acid solution(A) and aeetonitrile (B)with gradient elution program.The flow rate was 1 mL·min~(-1) and the column temperature was 25 ℃.The detection wavelength was 230 nm.Results:The average recovery was 98.2%,RSD=1.5 % (n=6) for paeoniflorin,the linear range was 1.749-17.49 μg·mL~(-1) (r=0.9996 ),and the average recovery was 97.4%,RSD=1.9% (n=6)for baicalin,the linera range was 13.33 -133.3 μg·mL~(-1)(r=0.9998).Conclusion:The method is simple,accurate and suitable for the determination of paeoniflorin and baicalin in Kuijiekang pepllets

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Objective:To establish a method for the determination of paeoniflorin and baicalin in Kujiekang peplle- ts.Methods:The HPLC method was conducted on a Kromasil C_(18)column(250 mm×4.6 mm,5.0 μm).The mobile phase consisted of 0.2% phosphoric acid solution(A) and aeetonitrile (B)with gradient elution program.The flow rate was 1 mL·min~(-1) and the column temperature was 25 ℃.The detection wavelength was 230 nm.Results:The average recovery was 98.2%,RSD=1.5 % (n=6) for paeoniflorin,the linear range was 1.749-17.49 μg·mL~(-1) (r=0.9996 ),and the average recovery was 97.4%,RSD=1.9% (n=6)for baicalin,the linera range was 13.33 -133.3 μg·mL~(-1)(r=0.9998).Conclusion:The method is simple,accurate and suitable for the determination of paeoniflorin and baicalin in Kuijiekang pepllets

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Available abstract

Objective:To establish a method for the determination of paeoniflorin and baicalin in Kujiekang peplle- ts.Methods:The HPLC method was conducted on a Kromasil C_(18)column(250 mm×4.6 mm,5.0 μm).The mobile phase consisted of 0.2% phosphoric acid solution(A) and aeetonitrile (B)with gradient elution program.The flow rate was 1 mL·min~(-1) and the column temperature was 25 ℃.The detection wavelength was 230 nm.Results:The average recovery was 98.2%,RSD=1.5 % (n=6) for paeoniflorin,the linear range was 1.749-17.49 μg·mL~(-1) (r=0.9996 ),and the average recovery was 97.4%,RSD=1.9% (n=6)for baicalin,the linera range was 13.33 -133.3 μg·mL~(-1)(r=0.9998).Conclusion:The method is simple,accurate and suitable for the determination of paeoniflorin and baicalin in Kuijiekang pepllets

Key concepts: Paeoniflorin, Baicalin, Chemistry, Chromatography, Phosphoric acid, High-performance liquid chromatography, Gradient elution, Linear range

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