Simultaneous Determination of Three Main Anti-inflammatory Constituents in Fuyankangfu Tablets by HPLC

Objective To establish a HPLC method for determining the contents of paeoniflorin,hesperidin and baicalin in Fuyankangfu Tablets. Methods HPLC was performed on a Phenomenex-C18 column(250 mm×4.60 mm,5 μm),and the mobile phase was composed of acetonitrile-0.05mol/L KH2PO4 (adjusted to pH 3.00 with H3PO4)with gradient elution(0 min,17∶83;10 min,17∶83;15 min,22∶78;30 min,30∶70).The flow-rate was 1.0 mL/min. The operating temperature was maintained at 40 ℃ and the detection wavelength was 231 nm. Results The linear ranges of paeoniflorin,hesperidin and baicalin were 0.0533～0.4800 μg(r=0.9997),0.0798～0.7182 μg(r=0.9999) and 0.3600～3.4200 μg (r=0.9999),respectively.The average recovery rate of paeoniflorin was 99.49%(RSD=1.70%,n=5),hesperidin was 99.59%(RSD=0.41%,n=5)and baicalin was 99.92%(RSD=1.26%,n=5).The contents of paeoniflorin in Fuyankangfu Tablets from three companies were 1.0656,1.4455,0.7042 mg,hesperidin were 0.5383,0.7676,0.4403 mg,baicalin were 3.9544,7.0312,2.8877 mg,respectively.Conclusion The method is simple,quick,accurate,which can be used for the quantity control of Fuyankangfu Tablets.