Surveillance of antibiotic resistance of clinically isolated bacteria in 2010

OBJECTIVE To master the popular hospital bacterial spectrum,review the result of the drug susceptibility testing so as to guide the clinical reasonable use of antibiotics and active control of nosocomial infections.METHODS The distribution and drug resistance of the pathogens isolated from various specimens submitted from Second Hospital of Shandong University in 2010 were analyzed,the bacterial identification was performed by using the VITEK identification system,disk diffusion method(KB method) was used for drug susceptibility testing,and microbiological testing Chinese statistical system ATBPLUS Ver3.3 was employed for data analysis.RESULTS Of 4105 strains of pathogens isolated,gram-negative bacteria were dominated,accounting for 57.25%;Acinetobacter baumannii was resistant to various antibiotics in general,the drug resistance rate to ampicillin was 99.13%;in contrast,the drug resistance rates of Pseudomonas aeruginosa to minocycline and sulfa drugs,the drug resistance rates to β-lactam,multiple peptide,aminoglycoside antibiotics were around 30.00%;Escherichia coli was resistant to β-lactam antibiotics in general,the resistance rate to cefoxitin was 12.04%,piperacillin/tazobactam 3.14%,cefoperazone/sulbactam 6.28%;Staphylococcus aureus was resistant to various antibiotics,the drug resistance rate to β-lactam antibiotics were higher than 90.00%,sulbactam/ampicillin 81.12%;no strains of vancomycin-resistant S.aureus were detected.CONCLUSION It is necessary to reasonably use antibiotics,strengthen the awareness of bacterial culture and strictly comply the standards of diagnosis so as to improve the quality of clinical anti-infection.