Tissue culture of wild'Qinling Yichang'lily

With wild'Qinling Yichang'lily as materials,effects of parts of scales and leaves on inducing adventitious buds,and effects of hormone combination on inducing adventitious buds,propagating buds and rooting were studied in order to presevve and propagate the wildlily resources.The results showed that the appropriate medium of the adventitious buds induced directly from scales was MS+2.0 mg/L 6-BA+0.05 mg/L NAA.The ability of scales inducing adventitious buds was downmiddleupper;the suitable medium of the buds induced from leaves was MS+1.0 mg/L 6-BA+0.1 mg/L NAA+2.0 mg/L 2,4-D.The induction rates of adventitious bud were down was high,middle and upper were low;the optimum proliferation medium for adventitious buds was MS+1.0 mg/L 6-BA+0.05 mg/L NAA.The appropriate medium for rooting was 1/2MS+0.4 mg/L NAA+1.0 g/L AC.The above study showed that there was obvious rule about inducing adventitious buds with different parts of scales and leaves,the combination of 6-BA and NAA had good effects on propagating buds,and the combination of NAA and AC was suitable for rooting.