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Study on Examination Seed Purity of Maize Hybrid by SSR Markers

Yuhu Zhang, Shuang Chen, Gao Shuren, Kejun Yang, Xia Wang, Yufeng Wang

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Abstract

In this study,used maize hybrids of Ken Yu6,Longdan-13 and parents as the materials to extract genomic DNA,and then examined hybrid seed purity by SSR markers.The results showed that the method of DNA extraction could obtain high quality DNA,which could be used for normal SSR amplification.Two pairs primers were screened out whose amplified bands showed parents complementary from 20 pairs of maize core primers,and the amplified bands were clear and identifiable.The purity of hybrid seeds Ken Yu6,Longdan-13 could be identified accurately.

About this research paper

What this paper is about

In this study,used maize hybrids of Ken Yu6,Longdan-13 and parents as the materials to extract genomic DNA,and then examined hybrid seed purity by SSR markers.The results showed that the method of DNA extraction could obtain high quality DNA,which could be used for normal SSR amplification.Two pairs primers were screened out whose amplified bands showed parents complementary from 20 pairs of maize core primers,and the amplified bands were clear and identifiable.The purity of hybrid seeds Ken Yu6,Longdan-13 could be identified accurately.

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Available abstract

In this study,used maize hybrids of Ken Yu6,Longdan-13 and parents as the materials to extract genomic DNA,and then examined hybrid seed purity by SSR markers.The results showed that the method of DNA extraction could obtain high quality DNA,which could be used for normal SSR amplification.Two pairs primers were screened out whose amplified bands showed parents complementary from 20 pairs of maize core primers,and the amplified bands were clear and identifiable.The purity of hybrid seeds Ken Yu6,Longdan-13 could be identified accurately.

Key concepts: Hybrid, DNA extraction, genomic DNA, DNA, Biology, Horticulture, Genetics, Polymerase chain reaction

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