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Application of SSR Markers in Hybrid Seed Purity Test of Maize

Li Xiao

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Abstract

The polymorphism of 21 maize elite inbred lines and 13 hybrids were analyzed with SSR marker system. Fifty eight out of 220 pairs of SSR primers were selected, which gave stable and polymorphic amplification profiles. Basically, the amplification bands of hybrids were complementary with that of the parents. Thirteen hybrids could be distinguished by combinations of two or three primers selected and statistical analysis. The rapid DNA extracting procedure from single kernel was established. The experiment showed that SSR technique could be effectively exploited in hybrid seed purity test of maize.

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What this paper is about

The polymorphism of 21 maize elite inbred lines and 13 hybrids were analyzed with SSR marker system. Fifty eight out of 220 pairs of SSR primers were selected, which gave stable and polymorphic amplification profiles. Basically, the amplification bands of hybrids were complementary with that of the parents. Thirteen hybrids could be distinguished by combinations of two or three primers selected and statistical analysis. The rapid DNA extracting procedure from single kernel was established. The experiment showed that SSR technique could be effectively exploited in hybrid seed purity test of maize.

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Available abstract

The polymorphism of 21 maize elite inbred lines and 13 hybrids were analyzed with SSR marker system. Fifty eight out of 220 pairs of SSR primers were selected, which gave stable and polymorphic amplification profiles. Basically, the amplification bands of hybrids were complementary with that of the parents. Thirteen hybrids could be distinguished by combinations of two or three primers selected and statistical analysis. The rapid DNA extracting procedure from single kernel was established. The experiment showed that SSR technique could be effectively exploited in hybrid seed purity test of maize.

Key concepts: Hybrid, Biology, Inbred strain, Genetics, Horticulture, Gene

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