Purification of total flavonoids from Valeriana jatamansi by macroporous adsorption resin

Objective To establish a method of purification process for total flavonoids from Valeriana jatamansi. Methods To select an optimum resin according to the adsorption rate and resolution rate from D-101, AB-8, HPD-600, and D-301, and study the factors such as adsorption amount, pH value, elution speed, concentration of adsorption, solution proportion, concentration of hydrochloric acid, concentration of ethanol, and amount of eluent, which could affect the adsorption and desorption behavior. Results The HPD 600 resin among four types of resins was found with the best separating efficiency. The best results of dynamic absorption were based on the followings: sample concentration was 3.03 mg/mL, feed rate 1 mL/min, and sample capacity 24.24 mg/g. After eluted with 0.1 mol/L HCl of 10% ethanol and 5 BV of 0.1 mol/L HCl of 80% ethanol. Conclusion HPD-600 resin could be used to purify the total flavonoids of V. jatamansi, and he yield of total flavones was 39.01% and the purity was 55.25%.