One–Way ANOVA Optimizing the Extract Technology of Amygdaloside in GZFL Capsules
Yanfen Huang
Abstract
Yanfen Huang
Abstract
Objective:To optimize the extract technology of amygdaloside in GZFL capsules.Methods:Take amygdaloside content in extract as observation index,One Way ANOVA was used to study the factor of concentration of ethanol to extract amygdaloside in GZFL capsules.GZFL was treated with deferent concentration ethanol.Amygdaloside was determinated by HPLC.The column was Hypersil C18(4.6 mm 150mm,5(m).The mobile phase consisted of methanol and 0.2% phosphoric acid(24∶76) at a flow rate of 1mL.min-1.The detection wavelength was 210nm.Results:Amygdaloside had a good linearity in the range of 0.3125-3.125μg(r=0.9999).The average recovery was 99.9%,RSD=0.36%.60% ethanol is the most suitable to extract Amygdaloside in GZFL capsules.Conclusion:The method is convenient,accurate,and has good reproducibility.It can be used to extract Amygdaloside in GZFL capsules.
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Objective:To optimize the extract technology of amygdaloside in GZFL capsules.Methods:Take amygdaloside content in extract as observation index,One Way ANOVA was used to study the factor of concentration of ethanol to extract amygdaloside in GZFL capsules.GZFL was treated with deferent concentration ethanol.Amygdaloside was determinated by HPLC.The column was Hypersil C18(4.6 mm 150mm,5(m).The mobile phase consisted of methanol and 0.2% phosphoric acid(24∶76) at a flow rate of 1mL.min-1.The detection wavelength was 210nm.Results:Amygdaloside had a good linearity in the range of 0.3125-3.125μg(r=0.9999).The average recovery was 99.9%,RSD=0.36%.60% ethanol is the most suitable to extract Amygdaloside in GZFL capsules.Conclusion:The method is convenient,accurate,and has good reproducibility.It can be used to extract Amygdaloside in GZFL capsules.
Key concepts: Chromatography, Phosphoric acid, Chemistry, Ethanol, Reproducibility, Content determination, Methanol, Ethanol content