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2010•Unpublished venueRequires access

Determination of icariin in Naoyiyuan capsules

Li Ming

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Abstract

Objective To determine icariin in Naoyiyuan capsules by HPLC.Methods HPLC was performed on Luna C18column(150 mm×4.6 mm,5 μm)with the mobile phase consisted of acetonitrile-water(30∶70)at a flow rate of 1.0 mL·min-1,and the detection wavelength was at 270 nm.Results Good linearity resulted in the concentration range of 0.069-0.483 μg,r=0.999 9.The average recovery was 95.39% with RSD of 2.5%(n=5).Conclusion This method is simple and accurate,and can be used for quality control of Naoyiyuan Capsules.

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What this paper is about

Objective To determine icariin in Naoyiyuan capsules by HPLC.Methods HPLC was performed on Luna C18column(150 mm×4.6 mm,5 μm)with the mobile phase consisted of acetonitrile-water(30∶70)at a flow rate of 1.0 mL·min-1,and the detection wavelength was at 270 nm.Results Good linearity resulted in the concentration range of 0.069-0.483 μg,r=0.999 9.The average recovery was 95.39% with RSD of 2.5%(n=5).Conclusion This method is simple and accurate,and can be used for quality control of Naoyiyuan Capsules.

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Available abstract

Objective To determine icariin in Naoyiyuan capsules by HPLC.Methods HPLC was performed on Luna C18column(150 mm×4.6 mm,5 μm)with the mobile phase consisted of acetonitrile-water(30∶70)at a flow rate of 1.0 mL·min-1,and the detection wavelength was at 270 nm.Results Good linearity resulted in the concentration range of 0.069-0.483 μg,r=0.999 9.The average recovery was 95.39% with RSD of 2.5%(n=5).Conclusion This method is simple and accurate,and can be used for quality control of Naoyiyuan Capsules.

Key concepts: Icariin, Chromatography, High-performance liquid chromatography, Chemistry, Recovery rate, Medicine, Pathology, Alternative medicine

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