UPLC determination of paeoniflorin in Sunanshandutong pills

Objective:To establish a ultra-performance liquid chromatography(UPLC) method for determination of paeoniflorin in Sunanshandutong pills.Methods:The separation was performed on Acquity BEH C18(2.1 mm×50 mm,1.7 μm) column;The mobile phase was acetonitrile-0.1% Phosphoric acid(15∶85) at a flow rate of 0.21 mL·min-1;The detection wavelength was 230 nm,and the column temperature was 25 ℃;Injection volume was 2 μL;Collection frequency was 20 Hz.Acquity Waters system was used for the assay.Results:Paeoniflorin was completely separated and detected in 4.5 minutes;Linear range was 0.026-0.264 μg(r=0.9999);Recovery(n=6) of method was 102.2%,RSD was 0.4%.Conclusion:The method is sensitive,accurate,precise,repeatable and suitable for determination of paeoniflorin in Sunanshandutong pills.Comparison to HPLC,UPLC obtain a faster analysis and less solvent consumption without reducing the efficiency.