The contrast and clinical acceptability estimation on the result of routine biochemistry items in different biochemical testing systems

Objective To study the results comparability of different biochemical testing systems by clinical acceptability estimation and method comparison.Methods According to National Committee for Clinical laboratory Standards(NCCLS)document EP9-A2,21 biochemistry items in different experimental(self-built) biochemical testing system and aim system were tested respectively to obtain the correlation coefficient and the linear equation.Then the system bias between experimental testing system and aim system could be evaluated by obtained equation.The comparability of different investigated system was judged according to the half of clinical laboratory improvement amendment 88(CLIA'88)allowed total error.Results The results of total protein(TP),alanine aminotransferase(ALT),alkaline phosphatase(ALP),direct bilimbin(DBil),Urea and inorganic phosphate(P)tested in system 1 and aim system showed no comparability.The results of glutamy itransferase(GGT),DBil,uric acid(UA),triglycefides(TG)and P tested in system 2 and aim system showed no comparability.There was comparability between system 3 and aim system in all items.Conclusion Routine biochemistry results had different bias between experimental(self-built) testing system and Roche Modular P-800 system.If the same item is tested in more than two systems.it is necessary to judge clinical acceptability by method comparison and clinical acceptability estimation so as to insure the comparability.