Comparative Study and Bias Estimation on the Results of Serum Enzyme with Different Biochemical Testing System Inter-hospital

Objective To study the comparability results of serum enzyme ALT,AST,ALP,GGT and AMS on different biochemical detecting systems inter-hospital through bias estimation and method comparison.Methods Following to CLSI document EP9-A2,we applied native blood samples and tested in different self-built biochemical testing systems and aim system respectively to obtain the correlation coefficient and the linear equation.The comparability of different investigating systems was judged according to the half of CLIA88 standard. Results (1)The correlation coefficient of GGT(system 3)and ALP(system4)were less than 0.975,which were not acceptable,the rest were acceptable.(2)The bias rate of ALT(system 1,4),ALP(system 3,5,6)and AMS(system 2,4) were higher than that of the half of CLIA'88 standard,which were not acceptable,the rest were acceptable.Conclusion There were several items were not acceptable in self-built system and it is necessary to do method comparison and bias estimation for judge clinical acceptability in order to insure the comparability.