ScholarLens Back to search
2006•Zhongguo shiyan fangjixue zazhiRequires access

Determination of Matrine and Oxymatrine in Sophora flavescens by HPLC

Tian Juan, Zhimin Wang

Open publisher page 0 citations

Abstract

Objective:To establish a high performance liquid chromatography(HPLC)method to determine the contents of matrine and oxymatrine in Sophora flavescens Ait.for selecting its suitable planting areas.Methods:A YWG-NH_2 was used as the solid phase,acetonitrile-3% phosphoric acid-ethand(80∶10∶10)as the mobile phase,with a detection wavelength at 220nm.Results:The calibration curves of matrine and oxymatrine were in good linearity over the rang of 0.050～0.061μg and 0.372～4.464μg,respectively.Conclusion:Sophora flavescens growing in Licheng,Zuoquan,Linfen in Shanxi province and Taibai in Shaanxi province contained higher oxymatrine than 3%.Content differences of matrine were smaller in different growing places.

About this research paper

What this paper is about

Objective:To establish a high performance liquid chromatography(HPLC)method to determine the contents of matrine and oxymatrine in Sophora flavescens Ait.for selecting its suitable planting areas.Methods:A YWG-NH_2 was used as the solid phase,acetonitrile-3% phosphoric acid-ethand(80∶10∶10)as the mobile phase,with a detection wavelength at 220nm.Results:The calibration curves of matrine and oxymatrine were in good linearity over the rang of 0.050～0.061μg and 0.372～4.464μg,respectively.Conclusion:Sophora flavescens growing in Licheng,Zuoquan,Linfen in Shanxi province and Taibai in Shaanxi province contained higher oxymatrine than 3%.Content differences of matrine were smaller in different growing places.

Why it matters

A significance statement is not available in the OpenAlex record.

Key contribution

A contribution statement is not available in the OpenAlex record.

Method / approach

Method details are not available in the OpenAlex metadata.

Main findings

Findings are not separately available in the OpenAlex metadata.

Limitations

Limitations are not available in the OpenAlex metadata.

Applications

Application details are not available in the OpenAlex metadata.

Available abstract

Objective:To establish a high performance liquid chromatography(HPLC)method to determine the contents of matrine and oxymatrine in Sophora flavescens Ait.for selecting its suitable planting areas.Methods:A YWG-NH_2 was used as the solid phase,acetonitrile-3% phosphoric acid-ethand(80∶10∶10)as the mobile phase,with a detection wavelength at 220nm.Results:The calibration curves of matrine and oxymatrine were in good linearity over the rang of 0.050～0.061μg and 0.372～4.464μg,respectively.Conclusion:Sophora flavescens growing in Licheng,Zuoquan,Linfen in Shanxi province and Taibai in Shaanxi province contained higher oxymatrine than 3%.Content differences of matrine were smaller in different growing places.

Key concepts: Matrine, Oxymatrine, Sophora flavescens, Sophora, High-performance liquid chromatography, Phosphoric acid, Chromatography, Chemistry

Related papers

Related paper 1Related paper 2Related paper 3
Back to paper searchBrowse research topicsOriginal source
Determination of Matrine and Oxymatrine in Sophora flavescens by HPLC — Research Paper | ScholarLens