Determination of Matrine and Oxymatrine in the Crude Extract of Sophora flavescens by HPLC

Objective To develop a method for determinition matrine and oxymatrine of crude extract of Sophora flavescens. Methods AichromBond-AQ C18 column(4.6 mm×250 mm,5 μm)was used as the solid phase.Methanol-0.05% triethylamine solution(70:30,V/V)was taken as mobile phase.The flow rate was 1.00 ml/min.The UV detection wavelength was 215 nm. Results The calibration curves of matrine and oxymatrine were linear in the range of 0.02~160 mg/L and 0.04~100 mg/L,respectively.The average recoveries of both were 98.59% and 99.43%,respectively. Conclusion The method established method is sensitive,simple,and accurate.