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HPLC assay of matrine and oxymatrine in total alkaloids from Sophora flavescens Ait.

Fan Chun-yan

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Abstract

Objective:To develop a method for determining the contents of matrine and oxymatrine in total alkaloids from Sophora flavescens Ait.Methods:A Elite Hypersil NH_2 column(5 μm,250 mm×4.6 mm)was used with the mobile phase being acetonitrile-absolute alcohol-3% phosphoric acid solution(81:10:9),flow rate being 1.0 mL·min~(-1),determinating wavelength being 220 nm,the column temperature being 25℃,The injection volume was 10 μL.Results:The calibration curves of matrine,oxymatrine were in good linearity over the ranges of 5.32- 53.2 μg·mL~(-1)(r=0.9997),65.7-525.6 μg·mL~(-1)(r=0.9995),and the average recovery of matrine and oxymatrine was 99.5 %,99.7 % with RSD of 1.0% ,0.6%(n=6)respectively.Conclusion:The method is simple, rapid,reliable and can be used for the total alkaloids in Sophora flavescens Ait.control.

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Objective:To develop a method for determining the contents of matrine and oxymatrine in total alkaloids from Sophora flavescens Ait.Methods:A Elite Hypersil NH_2 column(5 μm,250 mm×4.6 mm)was used with the mobile phase being acetonitrile-absolute alcohol-3% phosphoric acid solution(81:10:9),flow rate being 1.0 mL·min~(-1),determinating wavelength being 220 nm,the column temperature being 25℃,The injection volume was 10 μL.Results:The calibration curves of matrine,oxymatrine were in good linearity over the ranges of 5.32- 53.2 μg·mL~(-1)(r=0.9997),65.7-525.6 μg·mL~(-1)(r=0.9995),and the average recovery of matrine and oxymatrine was 99.5 %,99.7 % with RSD of 1.0% ,0.6%(n=6)respectively.Conclusion:The method is simple, rapid,reliable and can be used for the total alkaloids in Sophora flavescens Ait.control.

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Available abstract

Objective:To develop a method for determining the contents of matrine and oxymatrine in total alkaloids from Sophora flavescens Ait.Methods:A Elite Hypersil NH_2 column(5 μm,250 mm×4.6 mm)was used with the mobile phase being acetonitrile-absolute alcohol-3% phosphoric acid solution(81:10:9),flow rate being 1.0 mL·min~(-1),determinating wavelength being 220 nm,the column temperature being 25℃,The injection volume was 10 μL.Results:The calibration curves of matrine,oxymatrine were in good linearity over the ranges of 5.32- 53.2 μg·mL~(-1)(r=0.9997),65.7-525.6 μg·mL~(-1)(r=0.9995),and the average recovery of matrine and oxymatrine was 99.5 %,99.7 % with RSD of 1.0% ,0.6%(n=6)respectively.Conclusion:The method is simple, rapid,reliable and can be used for the total alkaloids in Sophora flavescens Ait.control.

Key concepts: Matrine, Oxymatrine, Sophora flavescens, Chemistry, Chromatography, Sophora, Phosphoric acid, High-performance liquid chromatography

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