Study on optimization of process conditions for enzymatic hydrolysis of cell protein of monascus with alkaline protease

To achieve more valuable utilization of cell filtered sediment of Monascus,the process conditions for enzymatic hydrolysis of cell protein of Monascus with alkaline protease was studied.Using the single factor and orthogonal design experiment,respectively,the optimum conditions of enzymatic hydrolysis were determined as follows: pH of 8.5,temperature of 55 ℃,concentration of substrate of 40 g/L,enzyme dosage of 6×104 U/g(cell amount),and enzymatic hydrolysis time of 20 hours.Under above optimum conditions,the enzymatic hydrolysis rate was 25.28%.