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2011•Journal of Henan University of TechnologyRequires access

OPTIMIZATION OF ENZYMATIC HYDROLYSIS CONDITIONS OF PEA PROTEIN BY NEUTRAL PROTEASE

Tingting Hu

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Abstract

We studied the enzymatic hydrolysis conditions of pea protein by using neutral protease.On the basis of singe-factor analysis,we adopted the response surface methodology to optimize the enzymatic hydrolysis conditions,and the optimum hydrolysis conditions were as follows: substrate concentration 0.515 g/mL,reaction time 3 hours,reaction temperature 54.6 ℃,pH 6.5 and enzyme-substrate ratio 2.95%.Under optimum conditions,the degree of hydrolysis of pea protein was 7.82%.

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What this paper is about

We studied the enzymatic hydrolysis conditions of pea protein by using neutral protease.On the basis of singe-factor analysis,we adopted the response surface methodology to optimize the enzymatic hydrolysis conditions,and the optimum hydrolysis conditions were as follows: substrate concentration 0.515 g/mL,reaction time 3 hours,reaction temperature 54.6 ℃,pH 6.5 and enzyme-substrate ratio 2.95%.Under optimum conditions,the degree of hydrolysis of pea protein was 7.82%.

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Available abstract

We studied the enzymatic hydrolysis conditions of pea protein by using neutral protease.On the basis of singe-factor analysis,we adopted the response surface methodology to optimize the enzymatic hydrolysis conditions,and the optimum hydrolysis conditions were as follows: substrate concentration 0.515 g/mL,reaction time 3 hours,reaction temperature 54.6 ℃,pH 6.5 and enzyme-substrate ratio 2.95%.Under optimum conditions,the degree of hydrolysis of pea protein was 7.82%.

Key concepts: Hydrolysis, Neutral protease, Chemistry, Protease, Enzyme, Substrate (aquarium), Enzymatic hydrolysis, Chromatography

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