Study on optimization of process conditions for enzymatic hydrolysis of cell protein of Monascus with neutrase

To achieve more valuable utilization of cell filtered sediment of Monascus,the process conditions for enzymatic hydrolysis of cell protein of Monascus with neutrase was studied.Using the single factor experiment and orthogonal design experiment respectively,the optimum conditions of enzymatic hydrolysis were determined as follows: pH of 6.5,temperature of 45 ℃,concentration of substrate of 35 g/L,enzyme dosage of 60000 U/g(cell amount),and enzymatic hydrolysis time of 16 hours.Under above optimum conditions,the hydrolysis degree of enzymatic process was 9.12%,and the total hydrolysis degree reached 14.73%.