Relativity of ELISA and TPPA methods in detection of treponema pallidum antibody

Objective: To investigate the relativity of enzyme-linked immunosorbent assay( ELISA) and treponema pallidum antibody gelatin particle agglutination test( TPPA) in detection of syphilis. Methods: Treponema pallidum antibodies in the serum were detected by ELISA,and a total of 207 positive samples and 50 negative samples were collected; then the collected samples were confirmed by TPPA test. According to the s /co value,the patients were divided into four groups and the positive rate of each group was analyzed by TPPA method. Results: The treponema pallidum antibody in the sera of 50 patients with s /co value 1 by ELISA were all negative when detected by TPPA; the positive rates of the serum treponema pallidum antibody in patients with s /co value of 1 to 3. 99,4 to 9. 99 and≥10 by ELISA were 88. 7%,98. 6% and 100. 0%,respectively. The positive rate of treponema pallidum antibody by TPPA test increased with the s /co value by ELISA method( P 0. 01). Conclusions: ELISA is a highly sensitive and automated method in detection of syphilis,which is suitable for clinical routine screening of a large number of specimens. Test by ELISA and TPPA is recommended when s /co value is between 1 and 9. 99 in syphilis detection by ELISA to reduce the false positive possibility and provide more accurate laboratory evidence for clinical diagnosis and treatment of syphilis.