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Determination content of total Saponins of dolichos tenuicaulis(Baker) Craib and crude extracts by spectrophotometry

Jinyong Peng

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Abstract

[Objective] To establish a method for determination of total saponins of dolichos tenuicaulis(Baker) Craib and crude extracts by UV.[Methods] The samples were determined by UV after reaction with vanillin-perchloric acid,and ginsenoside Rg1 was selected as the standard.Some parameters which might effect the results were all investigated and optimized.[Results] The suitable analytic conditions were optimized as follows: 5% vanillin-acetic acid solution 0.2 mL,perchloric acid 0.8 mL,temperature 60℃,reaction time 15 min.,detection wavelength 550 nm.The samples were linear in the range of 3.25~19.52 μg/mL with the regression equation of Y=0.030X+0.117(r=0.9995),and the average recovery was 97.45%(RSD=1.75%,n=5) under the selected conditions.[Conclusion] This method is suitable for the determination of total saponins for quality control of dolichos tenuicaulis(Baker) Craib.

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[Objective] To establish a method for determination of total saponins of dolichos tenuicaulis(Baker) Craib and crude extracts by UV.[Methods] The samples were determined by UV after reaction with vanillin-perchloric acid,and ginsenoside Rg1 was selected as the standard.Some parameters which might effect the results were all investigated and optimized.[Results] The suitable analytic conditions were optimized as follows: 5% vanillin-acetic acid solution 0.2 mL,perchloric acid 0.8 mL,temperature 60℃,reaction time 15 min.,detection wavelength 550 nm.The samples were linear in the range of 3.25~19.52 μg/mL with the regression equation of Y=0.030X+0.117(r=0.9995),and the average recovery was 97.45%(RSD=1.75%,n=5) under the selected conditions.[Conclusion] This method is suitable for the determination of total saponins for quality control of dolichos tenuicaulis(Baker) Craib.

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Available abstract

[Objective] To establish a method for determination of total saponins of dolichos tenuicaulis(Baker) Craib and crude extracts by UV.[Methods] The samples were determined by UV after reaction with vanillin-perchloric acid,and ginsenoside Rg1 was selected as the standard.Some parameters which might effect the results were all investigated and optimized.[Results] The suitable analytic conditions were optimized as follows: 5% vanillin-acetic acid solution 0.2 mL,perchloric acid 0.8 mL,temperature 60℃,reaction time 15 min.,detection wavelength 550 nm.The samples were linear in the range of 3.25~19.52 μg/mL with the regression equation of Y=0.030X+0.117(r=0.9995),and the average recovery was 97.45%(RSD=1.75%,n=5) under the selected conditions.[Conclusion] This method is suitable for the determination of total saponins for quality control of dolichos tenuicaulis(Baker) Craib.

Key concepts: Perchloric acid, Chemistry, Chromatography, Acetic acid, Vanillin, Spectrophotometry, Food science, Biochemistry

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