Chromatographic Separation of Capsaicinoids from the Extract of Capsicum Frutescens L.by Macroporous Adsorption Resin

Capsaicinoids was isolated and purified by 40% ethanol extraction, followed by macroporous adsorption resin column chromatography with 70% ethanol as elutent from extract of Capsicum frutescens L.. The concentration of ethanol, isolated capsaicinoids from pigment primarily, was studied. A solution contained capsaicinoids 0.42 g/L was obtained by extraction separation with 40% ethanol from the extract of Capsicum frutescens L. with a recovery ratio of 78%. A macroporous adsorption resin was selected as the medium for the purification of capsaicinoids after comparing static adsorption and elution performances of 11 kinds of macroporous adsorption resin and its static adsorption capacity was 26.1 mg/g. Further investigation of dynamic adsorption and elution behavior indicated that resin D01 had the capability of selective adsorption of capsaicinoids from the extract of Capsicum frutescens L. with 16.0 mg/g of dynamic adsorption capacity. The capsaicinoids, by extraction separation with 40% ethanol from the extract of Capsicum frutescens L. and then macroporous adsorption resin column chromatography with 70% ethanol as elutent, was enriched from 1.5% to 81.7% as determinated by HPLC, with a recovery ratio of 78.3%. It is simple and useful and meets the demands of green chemistry.