2013•Journal of Jiangxi University of Traditional Chinese MedicineRequires access

Determination of Hydroxysafflor Yellow A in Compound Shuxiong Dropping Pill by HPLC

WU Pe

Abstract

Objective To establish a detemination method for Hydroxysafflor yellow A in Compound Shuxiong Dropping Pill. Methods:The HPLC analysis was performed on a Shimadzu VP-ODS C18( 250 mm × 4.6 mm,5μm) column.The mobile phase consist ed of methyl-Acetonitrile-0.2%phosphoric acid(19:9:72,v/v) at the flow rate of 1.0 mL/min.The detected wavelength was 400 nm,and the column temperature was 25℃.Results:The standard curve for Hydroxysafflor yellow A showed a linear correlation in the range of 0.05136~1.0272mg(r=0.9999),the average recovery was 99.16% and RSD was 0.37%.Conclusion:The method is simple,accurate and suitable for the determination of Hydroxysafflor yellow A in Compound Shuxiong Dropping Pill.

About this research paper

What this paper is about

Why it matters

A significance statement is not available in the OpenAlex record.

Key contribution

A contribution statement is not available in the OpenAlex record.

Method / approach

Method details are not available in the OpenAlex metadata.

Main findings

Findings are not separately available in the OpenAlex metadata.

Limitations

Limitations are not available in the OpenAlex metadata.

Applications

Application details are not available in the OpenAlex metadata.

Available abstract

Key concepts: Phosphoric acid, Chromatography, High-performance liquid chromatography, Chemistry, Linear range, Linear relationship, Linear correlation, Analytical Chemistry (journal)