2008•Pharmaceutical Journal of Chinese People's Liberation ArmyRequires access

Determination of the Content of Hydroxysafflor Yellow A in Shuxiong Preparation by HPLC

Song Hong

Abstract

Aim To develop a method for the determination of hydroxysafflor yellow A in Shuxiong Tablets and Shuxiong Pellets by HPLC.Methods The Phenomenex Luna C18(4.6mm×250mm,5μm)column was used. The mobile phase consisted of ACN:0.05% phosphoric acid (15∶85).The flow rate was 1.0ml/min;the detection wavelength was 403nm and column temperature was 25℃. Results The calibration curve was linear (r=0.999 9) in the range of 2.65～26.50μg·ml-1 for hydroxysafflor yellow A. The average recoveries of hydroxysafflor yellow A in Shuxiong Tablets and Shuxiong Pellets were 99.4% and 100.5%,RSD was 1.6% and 2.0%,respectively.Conclusion The method is quick and convenient for the determination of hydroxysafflor yellow A in Shuxiong Tablets and Shuxiong Pellets.

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Key concepts: Chromatography, Phosphoric acid, Pellets, Calibration curve, Content determination, High-performance liquid chromatography, Chemistry, Linear range