ScholarLens Back to search
2011•Zhiye yu jiankangRequires access

Determination of Ursolic Acid in Hawthorn by RP-HPLC

Fang Wang

Open publisher page 0 citations

Abstract

[Objective] To find out the optimized process for ursolic acid and oleanolic acid in hawthorn,and establish the method for determination of ursolic acid in hawthorn herbs by RP-HPLC. [Methods]RP-HPLC method was adopted,with column C 18(250 mm×4.6 mm×5 μm),methanol-water-acetic acid as mobile phase(83∶17∶0.04),Column temperature: 25 ℃,flow rate: 1.0 ml / min,detection wavelength: 220 nm. [Results]Ursolic acid had good linearity in the range of 0.071 4 mg/ml to 4.284 mg/ml with r=0.999 7.The recoveries was 101.66%,RSD=1.70%. [Conclusion]The method has good precision with accurate and reliable results.

About this research paper

What this paper is about

[Objective] To find out the optimized process for ursolic acid and oleanolic acid in hawthorn,and establish the method for determination of ursolic acid in hawthorn herbs by RP-HPLC. [Methods]RP-HPLC method was adopted,with column C 18(250 mm×4.6 mm×5 μm),methanol-water-acetic acid as mobile phase(83∶17∶0.04),Column temperature: 25 ℃,flow rate: 1.0 ml / min,detection wavelength: 220 nm. [Results]Ursolic acid had good linearity in the range of 0.071 4 mg/ml to 4.284 mg/ml with r=0.999 7.The recoveries was 101.66%,RSD=1.70%. [Conclusion]The method has good precision with accurate and reliable results.

Why it matters

A significance statement is not available in the OpenAlex record.

Key contribution

A contribution statement is not available in the OpenAlex record.

Method / approach

Method details are not available in the OpenAlex metadata.

Main findings

Findings are not separately available in the OpenAlex metadata.

Limitations

Limitations are not available in the OpenAlex metadata.

Applications

Application details are not available in the OpenAlex metadata.

Available abstract

[Objective] To find out the optimized process for ursolic acid and oleanolic acid in hawthorn,and establish the method for determination of ursolic acid in hawthorn herbs by RP-HPLC. [Methods]RP-HPLC method was adopted,with column C 18(250 mm×4.6 mm×5 μm),methanol-water-acetic acid as mobile phase(83∶17∶0.04),Column temperature: 25 ℃,flow rate: 1.0 ml / min,detection wavelength: 220 nm. [Results]Ursolic acid had good linearity in the range of 0.071 4 mg/ml to 4.284 mg/ml with r=0.999 7.The recoveries was 101.66%,RSD=1.70%. [Conclusion]The method has good precision with accurate and reliable results.

Key concepts: Ursolic acid, Chromatography, Chemistry, Oleanolic acid, High-performance liquid chromatography, Acetic acid, Methanol, Biochemistry

Related papers

Related paper 1Related paper 2Related paper 3
Back to paper searchBrowse research topicsOriginal source
Determination of Ursolic Acid in Hawthorn by RP-HPLC — Research Paper | ScholarLens