2008•Zhongguo shiyan fangjixue zazhiRequires access

Determination of Ursolic Acid in Hawthorn Leaves by HPLC

LU Wen-sheng

Abstract

Objective:To develop an HPLC method for determination of ursolic acid in Hawthorn Leaves. Methods:The C_ 18 column with methanol-water-phosphorous acid (90∶10∶0.03) as the mobile phase was used. The flow rate was 0.5 mL·min~ -1 and the detection wavelength was 220 nm. Results:The linear range of calibration curve was (0.982～9.82) μg·mL~ -1 for ursolic acid (r= 0.999 8 ). The average recovery was 98.16% and the RSD was 0.58% (n=6). Conclusion:The method is simple, accurate and repeatable. It can be used for the quality control of Hawthorn Leaves.

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Key concepts: Ursolic acid, Chromatography, Calibration curve, High-performance liquid chromatography, Chemistry, Recovery rate, Linear relationship, Methanol