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EXTRACTION AND CONTENT ANALYSIS OF URSOLIC ACID FROM HAWTHORN

Liao Qing-yuan

Abstract

Established the contents determination of ursolic acid in hawthorn by high performance liquid chromatography. The chromatography conditions as follow: The liquid chromatography was carried out using a Intertex C18 with detection wavelength at 210 nm. The mobile phase was methanol-water(88:12). The colum temperature was 25 ℃. The flow rate was 1.0 mL/min. Check wavelength is 210 nm. The calibration curve of ursolic acid was linear in the range of 2.96 μg-8.88 μg (r=0.999 7). The average recovery was 98.29 % with RSD 0.38 %. The content of ursolic acid in Hawthorn was 0.321 %.

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Key concepts: Ursolic acid, Chromatography, Chemistry, High-performance liquid chromatography, Calibration curve, Recovery rate, Extraction (chemistry), Linear relationship