Determination of Oleanolic Acid and Ursolic Acid in Zizhu Fruit by High Performance Liquid Chromatography

Objective: To establish a high performance liquid chromatography(HPLC) method for determination of oleanolic acid and ursolic acid in Zizhu fruit.Methods: The HPLC method was carried on C18(4.6 mm×250 mm,5 μm)column using methanol-water(90∶10,V:V) as the mobile phase,and detection wavelength was 215 nm.The flow rate was 1.0 ml/min and the temperature of column 25 ℃.Results: The calibration curve for oleanolic acid was linear in the range of 0.011 5～0.103 5 g/L,and the calibration curve for ursolic acid was linear in the range of 0.030 32～0.272 88 g/L,The average recovery of oleanolic acid was 99.70%,and RSD was 0.32%(n=9),while average recovery of ursolic acid was 100.06%,RSD 0.87%(n=9).Conclusions: The method is convenient,precise,reliable and with good reproducibility for determining the contents of oleanolic acid and ursolic acid in Zizhu,which may provide basis for effective quality control of Zizhu fruit products.