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2013•Nonwood Forest ResearchRequires access

Cloning and bioinformatics analysis of LOX gene in Vernicia fordii seed

Xiaofeng Tan

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Abstract

In order to explore molecular mechanism of α-eleostearic acid biosynthesis in Vernicia fordii,a full-length cDNA of LOX gene was cloned by RT-PCR and RACE techniques,and the characters of LOX gene were analyzed by bioinformatics methods.The results show that full-length cDNA of LOX gene is 2 690 bp,and 797 amino acid are encoded.

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What this paper is about

In order to explore molecular mechanism of α-eleostearic acid biosynthesis in Vernicia fordii,a full-length cDNA of LOX gene was cloned by RT-PCR and RACE techniques,and the characters of LOX gene were analyzed by bioinformatics methods.The results show that full-length cDNA of LOX gene is 2 690 bp,and 797 amino acid are encoded.

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Available abstract

In order to explore molecular mechanism of α-eleostearic acid biosynthesis in Vernicia fordii,a full-length cDNA of LOX gene was cloned by RT-PCR and RACE techniques,and the characters of LOX gene were analyzed by bioinformatics methods.The results show that full-length cDNA of LOX gene is 2 690 bp,and 797 amino acid are encoded.

Key concepts: Cloning (programming), Gene, Complementary DNA, Biology, Molecular cloning, Rapid amplification of cDNA ends, Genetics, cDNA library

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