2009•Linye kexue yanjiuRequires access
Construction of Kernel cDNA Library and Bioinformatic Analysis on oleosins Gene of Vernicia fordii
Guan Zhou, Wang YangDong, Chen YiCun, Peng Li, Shanshan Zhang, Xiaoping Zhang
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Abstract
Seed kernel of Vernicia fordii was used as materials to construct a cDNA library at the key stage of fatty acids synthesis.A primary library of 1×106 pfu·mL-1 was obtained.The recombinant efficiency was 99.7%,and the sizes of inserted fractions ranged from 0.5 to 2.5 kb,with an average value of l kb.These sequences mainly involved oil synthesis related genes,seed development genes with respective abundance.And there are also many other genes to be unknown.Furthermore,bioinformatic analysis on oil body protein oleosins gene from V.fordii was done,which was firstly obtained from the cDNA library.