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2004•Zhongguo xiandai yixue/Zhongguo xiandai yixue zazhiRequires access

A rapid and simple method for extracting human genomic DNA from peripheral blood

Wang Long-wu

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Abstract

Objective: To establish a rapid,simple,efficient method for extracting human genomic DNA from peripheral blood.Methods: peripheral blood is lysed by adding a chaotropic agent NaI, then genomic DNA is directly extracteded with chloroform: isoamyl alcohol(24∶ 1).Results: The Genomic DNA extracted with this method is of large molecular weight and A 260/280=(1. 87±0.11),(31±3.12)μg DNA can be gained from per milliliter peripheral blood. The results of PCR and restriction endonucleases digestion for the DNA are good. Conclusions: The me thod is simple, rapid ,efficient and could be used to handle a largenumber of clinical samples.

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What this paper is about

Objective: To establish a rapid,simple,efficient method for extracting human genomic DNA from peripheral blood.Methods: peripheral blood is lysed by adding a chaotropic agent NaI, then genomic DNA is directly extracteded with chloroform: isoamyl alcohol(24∶ 1).Results: The Genomic DNA extracted with this method is of large molecular weight and A 260/280=(1. 87±0.11),(31±3.12)μg DNA can be gained from per milliliter peripheral blood. The results of PCR and restriction endonucleases digestion for the DNA are good. Conclusions: The me thod is simple, rapid ,efficient and could be used to handle a largenumber of clinical samples.

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Available abstract

Objective: To establish a rapid,simple,efficient method for extracting human genomic DNA from peripheral blood.Methods: peripheral blood is lysed by adding a chaotropic agent NaI, then genomic DNA is directly extracteded with chloroform: isoamyl alcohol(24∶ 1).Results: The Genomic DNA extracted with this method is of large molecular weight and A 260/280=(1. 87±0.11),(31±3.12)μg DNA can be gained from per milliliter peripheral blood. The results of PCR and restriction endonucleases digestion for the DNA are good. Conclusions: The me thod is simple, rapid ,efficient and could be used to handle a largenumber of clinical samples.

Key concepts: genomic DNA, Chaotropic agent, DNA, Peripheral blood, Lysis, Molecular biology, Isoamyl alcohol, DNA extraction

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