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2010•Laboratory Medicine and ClinicRequires access

An economical and effective method of extracting peripheral blood genomic DNA

Xiujun Li

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Abstract

Objective To introduce an economical and effective method for extracting human peripheral blood genomic DNA.Methods The leukocytes were isolated from human peripheral blood by 0.2% NaCl and then the genomic DNA was extracted from the leukocytes with a DNA extract kit.Results The reagents volume of extract 100 μL peripheral blood genomic DNA by original method could extract 3mL peripheral blood genomic DNA by new method,with the cost being saved by more than two-fold.Conclusion This method is very economical and effective,meanwhile it can also obtain a large number of genomic DNA.

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What this paper is about

Objective To introduce an economical and effective method for extracting human peripheral blood genomic DNA.Methods The leukocytes were isolated from human peripheral blood by 0.2% NaCl and then the genomic DNA was extracted from the leukocytes with a DNA extract kit.Results The reagents volume of extract 100 μL peripheral blood genomic DNA by original method could extract 3mL peripheral blood genomic DNA by new method,with the cost being saved by more than two-fold.Conclusion This method is very economical and effective,meanwhile it can also obtain a large number of genomic DNA.

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Available abstract

Objective To introduce an economical and effective method for extracting human peripheral blood genomic DNA.Methods The leukocytes were isolated from human peripheral blood by 0.2% NaCl and then the genomic DNA was extracted from the leukocytes with a DNA extract kit.Results The reagents volume of extract 100 μL peripheral blood genomic DNA by original method could extract 3mL peripheral blood genomic DNA by new method,with the cost being saved by more than two-fold.Conclusion This method is very economical and effective,meanwhile it can also obtain a large number of genomic DNA.

Key concepts: genomic DNA, Peripheral blood, DNA, Computational biology, Biology, Molecular biology, Genetics, Immunology

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