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A Rapid Method for Extracting Human Genomic DNA from Whole Blood

Zhang Zhi

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Abstract

Objective To establish a rapid simple,efficient method for extracting human genomic DNA from whole blood. Methods Whole blood is lysed by adding a chaotropic agent NaI,then genomic DNA is directly extracteded with chloroform:isoamyl alcohol(24:1). Results The Genomic DNA extracted with this method is of large molecular weight 33.5±3.55μg DNA can be gained from per milliliter whole blood.The results of PCR and restriction endonucleases digestion for the DNA are good. Conclusions The method is simple, rapid ,efficient and could be used to handle a large number of clinical samples.

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What this paper is about

Objective To establish a rapid simple,efficient method for extracting human genomic DNA from whole blood. Methods Whole blood is lysed by adding a chaotropic agent NaI,then genomic DNA is directly extracteded with chloroform:isoamyl alcohol(24:1). Results The Genomic DNA extracted with this method is of large molecular weight 33.5±3.55μg DNA can be gained from per milliliter whole blood.The results of PCR and restriction endonucleases digestion for the DNA are good. Conclusions The method is simple, rapid ,efficient and could be used to handle a large number of clinical samples.

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Available abstract

Objective To establish a rapid simple,efficient method for extracting human genomic DNA from whole blood. Methods Whole blood is lysed by adding a chaotropic agent NaI,then genomic DNA is directly extracteded with chloroform:isoamyl alcohol(24:1). Results The Genomic DNA extracted with this method is of large molecular weight 33.5±3.55μg DNA can be gained from per milliliter whole blood.The results of PCR and restriction endonucleases digestion for the DNA are good. Conclusions The method is simple, rapid ,efficient and could be used to handle a large number of clinical samples.

Key concepts: genomic DNA, Whole blood, Isoamyl alcohol, Chaotropic agent, DNA, Lysis, DNA extraction, Biology

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