Study on repairing effect and mechanism of ice king burn-scald ointment on deep grade II burn in rats

Objective To investigate repairing effect and mechanism of Ice king Burn-scald ointment on deep grade Ⅱ burn wounds in rats. Methods Burned skin wound was made in 60 wistar male rats in accordance with the literature. The rats were randomly divided into 3 groups: the burn-scald ointment treated group,the jingwanhong ointment treated group,the model group,with 20 rats in each group. Samples were collected after 1,3,7,13,21 days of burn to determine healing rates and serum levels of TNF-α content. Expression changes of glut-2 receptor were detected by immunofluorescence 4 days after burn. Results There were no significant difference in the healing rates of the wound among the 3 groups after 1 day(P 0. 05). The healing rates of the wound were significantly higher in the burn-scald ointment group and the model group(P 0. 01) after 7,11,13,and 21 days. Compared with model group,glut-2 receptor expression was increased in the burn-scald ointment group,and the difference was statistically significant(P 0. 01). Conclusion Ice king Burn-scald ointment shows a notable repairing effect on burn wound. The mechanism may be associated with decreased serum levels of TNF- α content and increased glut-2 receptor expression.