Determination of Aloe-emodin,Rhein,Emodin,Chrysophanol and Rheochrysidin in QING RE JIE DU FANG by HPLC

Objective:To develop an HPLC method for determination of aloe-emodin,rhein,emodin,chrysophanol and physcion in QING RE JIE DU entract.Methods: The analysis was carried out on a Kromasil C_18(250 mm×4.6 mm,5 μm)column eluted with a mobile phase of methanal-water containing 0.2% phosphoric acid solution(V∶V=85∶15).The flow rate was 1.0 mL·min-1 and column temperature was 35 ℃.The detection wavelength was set at 254 nm.Results: The calibration curve was linear within the range of 1.012 5～16.2,1.025～16.4,1.025～16.4,1.025～16.4 and 0.625～10 μg for aloe-emodin,rhein,emodin,chrysophanol and physcion,respectively.The average recoveries were 101.5%,99.2%,101.9%,101.5%,101.4% for the abore five conponents,respectively.Conclusion: This method is convenient,accurate and reproducible for determination of aloe-emodin,rhein,emodin,chrysophanol and phascion in QING RE JIE DU extraet.